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Vice President Vance is expected to travel to Islamabad soon to lead a U.S. delegation for a second round of peace talks with Iran as the countries' two-week ceasefire nears its expiration day.

But the prospects for renewed diplomacy remain unclear. Iranian state media say no Iranian delegations have traveled to Islamabad "so far." President Trump reiterated, in an interview with CNBC Tuesday, that he 

 the ceasefire expires Wednesday evening Eastern time.

Mohammad Bagher Qalibaf, Iran's chief negotiator and parliament speaker, accused the United States late Monday of forcing Iran to the negotiating table and 

 on the contrary, Iran has been preparing "to reveal new cards on the battlefield."

"We do not accept negotiations under the shadow of threats," Qalibaf wrote in a post on social media.

Officials in Islamabad say the city remains ready to host the talks, though Pakistan's government has given no formal indication about the status of its ongoing mediation efforts.

The first round of talks, held just over a week ago in Islamabad, ended without an agreement. Vance later accused Iran of refusing to accept Washington's terms on Iran's plans for nuclear enrichment.

Here are the main sticking points in the negotiations:

For officials in Washington, the main points of contention remain control over the Strait of Hormuz and the future of Iran's nuclear program.

The Trump administration has said it wants commercial shipping through the strategic waterway to be fully restored. Around 20% of the world's crude oil and natural gas passes through the narrow strait.

Since the start of the war on Feb. 28, Iran's main leverage over the U.S. has been its control of the strait, including the collection of tolls from commercial ships passing through it as a condition for reopening the waterway.

"The weaker party gains just by virtue of entering into a negotiation process," Mark Freeman, executive director of the Institute for Integrated Transitions, a peace and security think tank based in Spain, 

Iran's grip on the Strait of Hormuz has rattled global markets, 

The other big demand coming from Washington centers on Iran's nuclear program – and whether Tehran will accept limits on nuclear enrichment.

 Monday, a pro-MAGA radio talk show, President Trump said Iran would return to negotiations, but insisted the country "will not have a nuclear weapon."

"They're going to negotiate," Trump said. "And if they don't, they're going to see problems like they've never seen before."

Trump has consistently said that one of the main objectives of the war with Iran is to ensure that it never has a nuclear weapon. In his first interview after the fighting started, he 

said he believed Iran was "going to attack first"

 if the United States did not act. Trump did not cite intelligence to support that claim.

Vice President Vance said the first round of ceasefire talks held over a week ago broke down because Iran would not commit to forgoing a nuclear weapon.

"The simple fact is that we need to see an affirmative commitment that they will not seek a nuclear weapon, and they will not seek the tools that would enable them to quickly achieve a nuclear weapon," Vance said.

For Iran, Lebanon and end to U.S. blockade remain key demands

For Tehran, the key demands for extending the ceasefire include an end to the U.S. naval blockade of Iranian ports and guarantees that the fighting between Israel and Hezbollah will not resume.

 last week, pausing fighting between Israel's military and the Iran-backed Lebanese militant group Hezbollah. That ceasefire is still holding.

Iran had earlier refused to engage in more negotiations with the United States unless Israel stopped its attacks on Hezbollah in Lebanon.

Israel, however, has vowed to keep its troops in southern Lebanon, and Hezbollah said the Lebanese people maintain the "right to resist" Israeli forces in the country.

Fighting between Israel and Hezbollah broke out shortly after the start of the war with Iran on Feb. 28, with Hezbollah firing rockets into northern Israel in a show of solidarity with Tehran. Israeli forces responded with airstrikes and an invasion of southern Lebanon. Israel 

 a "buffer zone" to distance Hezbollah fire from Israeli border communities.

Israel's strikes have killed more than 2,300 people and displaced over 1 million in Lebanon, according to Lebanese authorities. Hezbollah's attacks have killed at least 12 Israeli soldiers and two civilians, according to Israeli authorities.

As for the U.S. blockade of Iranian ports, Iran has made it clear it sees lifting the blockade as a condition for further diplomacy.

After the first round of ceasefire talks in Islamabad ended without an agreement, the U.S. military blockaded Iranian shipping ports, in a bid to pressure Iran to reopen the Strait of Hormuz as well as put economic pressure on Iran to end the war. The U.S. military says it has 

 to turn back since the blockade began. However, maritime data analysis firm Lloyd's List says it has tracked "

" vessels moving in and out of Iranian ports.

 over the strait once more in response to the continued U.S. blockade.

Iranian officials have also signaled they want financial relief, 

including access to $6 billion in frozen assets

, and do not want to negotiate while Washington keeps up military and economic pressure.

Rebecca Rosman contributed to this report from London.

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The status of the Strait of Hormuz and Iran's nuclear enrichment program are among the biggest obstacles to extending the truce, which expires Wednesday evening.

As the U.S.-Iran ceasefire deadline looms, here are the main sticking points

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